DNA binding, DNA cleavage and BSA interaction of Vanadium-Morin complex

Several oxovanadium(V) complexes have been shown as a potent anticancer agents. In view of the importance of Vanadium complexes a new Vanadium – Morin complex was synthesized and comprehensively characterized. To provide an insight on the mode and affinity of the interaction of these complex with calf thymus (CT) DNA the following experiments were carried out: fluorescence spectroscopy, UV–vis absorption, viscosity and circular dichoroic measurements. The cleavage properties of these complex with super coiled pBR322 DNA have been studied using the gel electrophoresis method, where the complex displayed chemical nuclease activity in presence of H2O2, presumably via an oxidative mechanism. These experimental results indicate that the complex interact through stacking between the base pairs of double helix DNA and the binding constants (Kb) of the synthesized complex was found to be 6.6 x 10 4 M. In addition, the DNA cleavage activity of the complex was determined using agarose gel electrophoresis and the results show that complexes have potent nuclease activity.